Whole Genome Sequencing (Cat. No. NGS-WGS-00x)

Introduction

De novo sequencing is typically performed without prior knowledge of the sequencing data. De novo sequencing has proven successful in confirming and expanding upon results from database searches, providing excellent resources for understanding a species. Some of the most crucial information, obtained by resequencing of an organism's genome DNA, are the individual variations in the genome, such as single nucleotide polymorphism (SNP), copy number variation (CNV), and structural variation (SV).

Sequencing Platforms:

• NovaSeq 6000, HiSeq X Ten, HiSeq 2500, NextSeq 500, MiSeq
• Sequel I, IIe
• DNBSEQ-T7, DNBSEQ-G400
• GridION. PromethION

Sample Type:

• Tissue
• cell
• whole blood (EDTA tube)
• plant
• etc.
• Contact our sales person regards to sample preparation and questions about sequencing procedure

Sample Requirement:

•  at least 1ug total gDNA

Data Analysis:

De novo Sequencing

• Standard Data Analysis
  - Consensus sequence assembled into contigs
  - De novo assembly, Error Correction, Gap Filling
  
  
• Advanced Data Dnalysis
  - 3 genome comparison, Gene Prediction & Annotation, Orthologous gene comparison, Pangenome Analysis, Whole genome alignment
  
  
  

Resequencing

• Standard Data Analysis
  - Mapping
  - Variant Calling(SNPs / lnDels)
  - CNV(control-FREEC)
  - SV(BreakDancer)
• Advanced Data Analysis
  -A variety of options for mapping algorithms, variant detection algorithms, annotations, mapping to public databases